Conventional synthetic packaging materials, predominantly non-biodegradable polymers, fail to effectively address food spoilage while contributing to environmental pollution. To overcome these limitations, active packaging materials have emerged as a promising strategy for extending shelf life and maintaining the quality of consumables. Electrospinning, a cost-effective and versatile technique for producing nonwoven polymer fibers, in the micro-to-nanometric range, enables the incorporation of functional agents such as lignin nanoparticles, known for their antioxidant, antimicrobial, and UV-blocking properties. By utilizing a self-developed three-dimensional electrospinning system, highly uniform polyvinyl alcohol mats with homogeneously dispersed lignin nanoparticles were fabricated, enhancing the potential of electrospun bio-based materials to reduce food spoilage, offering a viable alternative to conventional packaging.